分类： 生物学 >> 病毒学 提交时间： 2020-04-19
Shu, Ting
Huang, Muhan
Wu, Di
Ren, Yujie
Zhang, Xueyi
Han, Yang
Mu, Jingfang
Wang, Ruibing
Qiu, Yang
Zhang, Ding-Yu
Zhou, Xi
摘要： The ongoing outbreak of Coronavirus Disease 2019 (COVID-19) has become a global pandemic and crisis. SARS-coronavirus-2 (SARS-CoV-2), the causative pathogen of COVID-19, is a positive-sense single-stranded RNA virus belonging to the family Coronaviridae. For RNA viruses, virus-encoded RNA helicases have long been recognized to play pivotal roles during viral life cycles by facilitating the correct folding and replication of viral RNAs. Here, our studies show that SARS-CoV-2-encoded nonstructural protein 13 (nsp13) possesses the nucleoside triphosphate hydrolase (NTPase) and RNA helicase activities that can hydrolyze all types of NTPs and unwind RNA helices dependently of the presence of NTP, and further characterize the biochemical characteristics of these two enzymatic activities associated with SARS-CoV-2 nsp13. Moreover, we found that some bismuth salts could effectively inhibit both the NTPase and RNA helicase activities of SARS-CoV-2 nsp13 in a dose-dependent manner. Thus, our findings demonstrate the NTPase and helicase activities of SARS-CoV-2 nsp13, which may play an important role in SARS-CoV-2 replication and serve as a target for antivirals.
分类： 生物学 >> 病毒学 提交时间： 2020-02-22
Mu, Jingfang
Xu, Jiuyue
Shu, Ting
Wu, Di
Huang, Muhan
Ren, Yujie
Li, Xufang
Geng, Qing
Xu, Yi
Qiu, Yang
Zhou, Xi
摘要： 新型冠状病毒肺炎目前仍在武汉和中国其他地区持续。在目前的形势下，进一步了解新型冠状病毒（SARS-CoV-2）的病毒学和病毒与宿主的相互作用，对控制感染、开发有效的治疗方法具有重要意义。RNA干扰（RNA interference，RNAi）是一种进化上保守的真核生物抗病毒免疫机制，目前已发现许多病毒编码自身的RNA干扰抑制因子作为对抗措施。在这项研究中，我们发现SARS-CoV-2编码的核衣壳蛋白（N）有效地抑制了shRNAs或siRNAs触发的RNAi。此外，与许多由其他病毒编码的VSR类似，SARS-CoV-2的VSR在体外与人类细胞中与dsRNA相互作用，显示出双链RNA（dsRNA）结合活性。综上所述，我们的研究结果表明，SARS-CoV-2的N蛋白在人类细胞中表现出VSR活性，这可能是新型冠状病毒的一个关键免疫逃避因子。
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